Citrine
Citrine (Ex-Max 512 nm/Em-Max 528 nm)
<html>Citrine is a weakly dimeric green fluorescent protein with excitation and emission maximums of 516 nm and 528 nm, respectively. Citrine was first derived from <i>Aequorea victoria and is a mutation of EYFP. </i></html>
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 528 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Extinction coefficient
- 77000
- Quantum yield
- 0.76
- Molecular weight
- 27000
Citrine has an excitation maximum at 516 nm and an emission maximum at 528 nm, a Stokes shift of 12 nm. Its extinction coefficient is 77000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Citrine?
- <html>Citrine is a weakly dimeric green fluorescent protein with excitation and emission maximums of 516 nm and 528 nm, respectively. Citrine was first derived from <i>Aequorea victoria and is a mutation of EYFP. </i></html>
- What laser excites Citrine?
- Citrine is optimally excited by the 488 laser line. Its excitation maximum is 516 nm.
- What filter should I use to detect Citrine?
- A 515/30 filter captures the peak of the Citrine emission near 528 nm with minimal spillover.