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Venus
Venus (Ex-Max 515 nm/Em-Max 528 nm)
Venus is a weakly dimeric fluorescent protein originally derived from the jellyfish, Aequorea victoria. It has an excitation peak at 515 nm and an emission peak at 528 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 515 nm
- Emission maximum
- 528 nm
- Optimal laser line
- 488
- Optimal filter
- 525/30
- Extinction coefficient
- 92200
- Quantum yield
- 0.57
- Molecular weight
- 26800
Venus has an excitation maximum at 515 nm and an emission maximum at 528 nm, a Stokes shift of 13 nm. Its extinction coefficient is 92200 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Venus?
- Venus is a weakly dimeric fluorescent protein originally derived from the jellyfish, Aequorea victoria. It has an excitation peak at 515 nm and an emission peak at 528 nm.
- What laser excites Venus?
- Venus is optimally excited by the 488 laser line. Its excitation maximum is 515 nm.
- What filter should I use to detect Venus?
- A 525/30 filter captures the peak of the Venus emission near 528 nm with minimal spillover.