DMAO
DMAO (Ex-Max 497 nm/Em-Max 528 nm)
DMAO from Biotium is a cell-permeant fluorogenic DNA dye useful in staining gram positive and gram negative bacteria. It has an excitation peak at 497 nm and an emission peak at 528 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 528 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
DMAO has an excitation maximum at 497 nm and an emission maximum at 528 nm, a Stokes shift of 31 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is DMAO?
- DMAO from Biotium is a cell-permeant fluorogenic DNA dye useful in staining gram positive and gram negative bacteria. It has an excitation peak at 497 nm and an emission peak at 528 nm.
- What laser excites DMAO?
- DMAO is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect DMAO?
- A 525/50 filter captures the peak of the DMAO emission near 528 nm with minimal spillover.