LysoView 488
LysoView™ 488 (Ex-Max 497 nm/Em-Max 528 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 488 has an excitation peak at 497 nm and an emission peak at 528 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 528 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Commercial antibodies
- 2 from 1 supplier
LysoView 488 has an excitation maximum at 497 nm and an emission maximum at 528 nm, a Stokes shift of 31 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is LysoView 488?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 488 has an excitation peak at 497 nm and an emission peak at 528 nm.
- What laser excites LysoView 488?
- LysoView 488 is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect LysoView 488?
- A 525/50 filter captures the peak of the LysoView 488 emission near 528 nm with minimal spillover.
- How many antibodies are available conjugated to LysoView 488?
- FluoroFinder tracks 2 commercially available LysoView 488 conjugates, searchable by target, clone, host and application.