FVS510 (Fixable Viability Stain 510) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 416 nm and an emission peak at 506 nm.is used as a is used as a flow cytometry viability dye to determine cellular viability in mammalian cells.

What laser excites FVS510?

FVS510 is optimally excited by the 405 laser line. Its excitation maximum is 416 nm.

What filter should I use to detect FVS510?

A 515/30 filter captures the peak of the FVS510 emission near 506 nm with minimal spillover.

How many antibodies are available conjugated to FVS510?

FluoroFinder tracks 1 commercially available FVS510 conjugates, searchable by target, clone, host and application.

FVS510 Dye Profile | FluoroFinder

Excitation & emission spectra

Key facts

Excitation maximum: 416 nm
Emission maximum: 506 nm
Optimal laser line: 405
Optimal filter: 515/30
Commercial antibodies: 1 from 1 supplier

FVS510 has an excitation maximum at 416 nm and an emission maximum at 506 nm, a Stokes shift of 90 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is FVS510?: FVS510 (Fixable Viability Stain 510) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 416 nm and an emission peak at 506 nm.is used as a is used as a flow cytometry viability dye to determine cellular viability in mammalian cells.
What laser excites FVS510?: FVS510 is optimally excited by the 405 laser line. Its excitation maximum is 416 nm.
What filter should I use to detect FVS510?: A 515/30 filter captures the peak of the FVS510 emission near 506 nm with minimal spillover.
How many antibodies are available conjugated to FVS510?: FluoroFinder tracks 1 commercially available FVS510 conjugates, searchable by target, clone, host and application.