Live/Dead Fix Lime
Live/Dead Fix Lime (Ex-Max 408 nm/Em-Max 504 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Lime has an excitation peak at 408 nm and an emission peak at 504 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 408 nm
- Emission maximum
- 504 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Lime has an excitation maximum at 408 nm and an emission maximum at 504 nm, a Stokes shift of 96 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Live/Dead Fix Lime?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Lime has an excitation peak at 408 nm and an emission peak at 504 nm.
- What laser excites Live/Dead Fix Lime?
- Live/Dead Fix Lime is optimally excited by the 405 laser line. Its excitation maximum is 408 nm.
- What filter should I use to detect Live/Dead Fix Lime?
- A 515/30 filter captures the peak of the Live/Dead Fix Lime emission near 504 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Lime?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Lime conjugates, searchable by target, clone, host and application.