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eFluor 506 Fix Viability

eFluor™ 506 Fix Viability (Ex-Max 409 nm/Em-Max 503 nm)

eFluor™ 506 Fixable Viability from Thermo Fisher Scientific is used to irreversibly label dead cells prior cryopreservation, fixation, or permeabilization.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
409 nm
Emission maximum
503 nm
Optimal laser line
405
Optimal filter
515/30

eFluor 506 Fix Viability has an excitation maximum at 409 nm and an emission maximum at 503 nm, a Stokes shift of 94 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is eFluor 506 Fix Viability?
eFluor™ 506 Fixable Viability from Thermo Fisher Scientific is used to irreversibly label dead cells prior cryopreservation, fixation, or permeabilization.
What laser excites eFluor 506 Fix Viability?
eFluor 506 Fix Viability is optimally excited by the 405 laser line. Its excitation maximum is 409 nm.
What filter should I use to detect eFluor 506 Fix Viability?
A 515/30 filter captures the peak of the eFluor 506 Fix Viability emission near 503 nm with minimal spillover.