TO-Pro 1
TO-Pro 1 (Ex-Max 515 nm/Em-Max 531 nm)
TO-PRO-1 (Thiazole Orange Iodide) is an intercalating cell-impermeant nucleic acid stain used for viablity or apoptosis detection. It has an excitation peak at 512 nm and an emission peak at 533 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 515 nm
- Emission maximum
- 531 nm
- Optimal laser line
- 488
- Optimal filter
- 525/30
- Commercial antibodies
- 1 from 1 supplier
TO-Pro 1 has an excitation maximum at 515 nm and an emission maximum at 531 nm, a Stokes shift of 16 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is TO-Pro 1?
- TO-PRO-1 (Thiazole Orange Iodide) is an intercalating cell-impermeant nucleic acid stain used for viablity or apoptosis detection. It has an excitation peak at 512 nm and an emission peak at 533 nm.
- What laser excites TO-Pro 1?
- TO-Pro 1 is optimally excited by the 488 laser line. Its excitation maximum is 515 nm.
- What filter should I use to detect TO-Pro 1?
- A 525/30 filter captures the peak of the TO-Pro 1 emission near 531 nm with minimal spillover.
- How many antibodies are available conjugated to TO-Pro 1?
- FluoroFinder tracks 1 commercially available TO-Pro 1 conjugates, searchable by target, clone, host and application.