Thiazole Orange
Thiazole Orange (Ex-Max 512 nm/Em-Max 531 nm)
Thiazole Orange (TO) is a cell semi-permeant, fluorogenic nucleic acid stain with an affinity for RNA in blood cells and as a counterstain in live bacteria. It has an excitation peak at 512 nm and an emission peak at 533 nm. Thiazole Orange is commonly used to detect nucleic acids in solution and in DNA gels.
Excitation & emission spectra
Key facts
- Excitation maximum
- 512 nm
- Emission maximum
- 531 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Molecular weight
- 476.61
- Commercial antibodies
- 1 from 1 supplier
Thiazole Orange has an excitation maximum at 512 nm and an emission maximum at 531 nm, a Stokes shift of 19 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Thiazole Orange?
- Thiazole Orange (TO) is a cell semi-permeant, fluorogenic nucleic acid stain with an affinity for RNA in blood cells and as a counterstain in live bacteria. It has an excitation peak at 512 nm and an emission peak at 533 nm. Thiazole Orange is commonly used to detect nucleic acids in solution and in DNA gels.
- What laser excites Thiazole Orange?
- Thiazole Orange is optimally excited by the 488 laser line. Its excitation maximum is 512 nm.
- What filter should I use to detect Thiazole Orange?
- A 525/50 filter captures the peak of the Thiazole Orange emission near 531 nm with minimal spillover.
- How many antibodies are available conjugated to Thiazole Orange?
- FluoroFinder tracks 1 commercially available Thiazole Orange conjugates, searchable by target, clone, host and application.