About Thiazole Orange

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Thiazole Orange (TO) is a cell semi-permeant, fluorogenic nucleic acid stain with an affinity for RNA in blood cells and as a counterstain in live bacteria. It has an excitation peak at 512 nm and an emission peak at 533 nm. Thiazole Orange is commonly used to detect nucleic acids in solution and in DNA gels.

Alternative Dyes to Thiazole Orange

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Nuclear-ID Green Thiazole Orange Vybrant DyeCycle Green

Dyes similar to Thiazole Orange based on emission

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2-NBDG Acridine Orange Alexa Fluor 514 Amethyst Orange ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 Calcium Green CellBrite Steady 488 CellTrace Oregon Green CF450 CF503R Chromeo 505 Citrine CytoTrack Green DCF DMAO eFluor 520 Fix Viability EvaGreen EvaGreen Plus eYFP FITC Fluo-3 AM Fluo-3 GelGreen GOLGI-ID Green HiLyte 488 Krome Orange LIVE 510 Live/Dead Fix Aqua Live/Dead Fix Green Lucifer Yellow LysoSensor Yellow/Blue pH3.0 LysoView 488 mAmetrine mCitrine MitoLite Green EX488 MitoView Green NBD Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucView 488 Oregon Green 488 Oregon Green 514 Oxazole Gold PhenoVue Fluo-4 AM, Calcium Indicator pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) Qdot 525 ReadiLink 405-537 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spark Blue 550 Spark Violet 538 STAR 488 STAR 512 Syto 16 Sytox Green Thiazole Green Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 Venus ViaFluor 488 Vio Bright FITC Vybrant DyeCycle Green YFP RayBright LIVE 488 Spark PLUS B550 cFluor B532 AccuGreen HS ATTO Rho110 ATTO 495 RayBright Violet 500 BactoSpore 488/540 Nucleic Acid Stain ThiolTracker Violet Aluora 514