TOTO-1
TOTO-1 (Ex-Max 514 nm/Em-Max 533 nm)
TOTO-1 (Thiazole Orange Homodimer) from Thermo Fisher Scientific is a bis-intercalating, fluorogenic DNA dye used for counterstaining fixed cell nucleii or for assessing viability in flow cytometry. It has an excitation peak at 514 nm and an emission peak at 533 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 514 nm
- Emission maximum
- 533 nm
- Optimal laser line
- 488
- Optimal filter
- 525/30
- Commercial antibodies
- 1 from 1 supplier
TOTO-1 has an excitation maximum at 514 nm and an emission maximum at 533 nm, a Stokes shift of 19 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is TOTO-1?
- TOTO-1 (Thiazole Orange Homodimer) from Thermo Fisher Scientific is a bis-intercalating, fluorogenic DNA dye used for counterstaining fixed cell nucleii or for assessing viability in flow cytometry. It has an excitation peak at 514 nm and an emission peak at 533 nm.
- What laser excites TOTO-1?
- TOTO-1 is optimally excited by the 488 laser line. Its excitation maximum is 514 nm.
- What filter should I use to detect TOTO-1?
- A 525/30 filter captures the peak of the TOTO-1 emission near 533 nm with minimal spillover.
- How many antibodies are available conjugated to TOTO-1?
- FluoroFinder tracks 1 commercially available TOTO-1 conjugates, searchable by target, clone, host and application.