pHrodo Green
pHrodo Green (Ex-Max 509 nm/Em-Max 533 nm)
pHrodo Green from Thermo Fisher Scientific is a fluorogenic, cell-permeant pH indicator that is non-fluorescent at a neutral pH and increases in intensity with an increase in acidity involved in endocytosis and phagocytosis. PHrodo is often used to label proteins or biomolecules to track this process. PHrodo Green has an excitation peak at 509 nm and an emission peak at 533 nm, making it useful in both flow cytometry and fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 509 nm
- Emission maximum
- 533 nm
- Optimal laser line
- 488
- Optimal filter
- 525/30
- Commercial antibodies
- 1 from 1 supplier
pHrodo Green has an excitation maximum at 509 nm and an emission maximum at 533 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is pHrodo Green?
- pHrodo Green from Thermo Fisher Scientific is a fluorogenic, cell-permeant pH indicator that is non-fluorescent at a neutral pH and increases in intensity with an increase in acidity involved in endocytosis and phagocytosis. PHrodo is often used to label proteins or biomolecules to track this process. PHrodo Green has an excitation peak at 509 nm and an emission peak at 533 nm, making it useful in both flow cytometry and fluorescence microscopy.
- What laser excites pHrodo Green?
- pHrodo Green is optimally excited by the 488 laser line. Its excitation maximum is 509 nm.
- What filter should I use to detect pHrodo Green?
- A 525/30 filter captures the peak of the pHrodo Green emission near 533 nm with minimal spillover.
- How many antibodies are available conjugated to pHrodo Green?
- FluoroFinder tracks 1 commercially available pHrodo Green conjugates, searchable by target, clone, host and application.