Rhodamine 123
Rhodamine 123 (Ex-Max 512 nm/Em-Max 531 nm)
Rhodamine 123 is a generic fluorophore that has an excitation peak at 512 nm and an emission peak at 531 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 512 nm
- Emission maximum
- 531 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Extinction coefficient
- 85200
- Quantum yield
- 0.9
- Molecular weight
- 381
Rhodamine 123 has an excitation maximum at 512 nm and an emission maximum at 531 nm, a Stokes shift of 19 nm. Its extinction coefficient is 85200 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Rhodamine 123?
- Rhodamine 123 is a generic fluorophore that has an excitation peak at 512 nm and an emission peak at 531 nm.
- What laser excites Rhodamine 123?
- Rhodamine 123 is optimally excited by the 488 laser line. Its excitation maximum is 512 nm.
- What filter should I use to detect Rhodamine 123?
- A 525/50 filter captures the peak of the Rhodamine 123 emission near 531 nm with minimal spillover.