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PKH67

PKH67 (Ex-Max 490 nm/Em-Max 502 nm)

PKH67 is a ampiphilic dye used to intercalate into bioplar membranes and has an excitation peak at 490 nm and an emission peak at 502 nm. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
490 nm
Emission maximum
502 nm
Optimal laser line
488
Optimal filter
515/30

PKH67 has an excitation maximum at 490 nm and an emission maximum at 502 nm, a Stokes shift of 12 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is PKH67?
PKH67 is a ampiphilic dye used to intercalate into bioplar membranes and has an excitation peak at 490 nm and an emission peak at 502 nm. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
What laser excites PKH67?
PKH67 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
What filter should I use to detect PKH67?
A 515/30 filter captures the peak of the PKH67 emission near 502 nm with minimal spillover.