Amethyst Orange
Amethyst Orange (Ex-Max 390 nm/Em-Max 535 nm)
Amethyst Orange from Bio-Rad has an excitation peak at 390 nm and an emission peak at 535 nm. It can be excited by the 405 nm laser on a flow cytometer and is spectrally most similar to Pacific Orange. However, StarBright Violet 515 and StarBright Violet 570 both offer brighter alternatives for that emission range in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 390 nm
- Emission maximum
- 535 nm
- Optimal laser line
- 405
- Optimal filter
- 525/50
- Commercial conjugates
- 3 from 1 supplier
Amethyst Orange has an excitation maximum at 390 nm and an emission maximum at 535 nm, a Stokes shift of 145 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Amethyst Orange?
- Amethyst Orange from Bio-Rad has an excitation peak at 390 nm and an emission peak at 535 nm. It can be excited by the 405 nm laser on a flow cytometer and is spectrally most similar to Pacific Orange. However, StarBright Violet 515 and StarBright Violet 570 both offer brighter alternatives for that emission range in flow cytometry.
- What laser excites Amethyst Orange?
- Amethyst Orange is optimally excited by the 405 laser line. Its excitation maximum is 390 nm.
- What filter should I use to detect Amethyst Orange?
- A 525/50 filter captures the peak of the Amethyst Orange emission near 535 nm with minimal spillover.
- How many antibodies are available conjugated to Amethyst Orange?
- FluoroFinder tracks 3 commercially available Amethyst Orange conjugates, searchable by target, clone, host and application.