About Spark Blue 515

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Spark Blue™ 515 exhibits peak excitation/emission wavelengths at 506 nm and 516 nm respectively, most closely matching the spectra of cFluor® B515. On conventional cytometers, it can be detected with a 488 nm blue laser and the 530/30 filter set typically used by FITC or Alexa Fluor® 488. On spectral instruments with the proper settings, it is possible to unmix Spark Blue™ 515 from dyes like FITC, Alexa Fluor® 488, and KIRAVIA Blue 520™ as its peak emission is shifted over 10 nm. With a dim brightness, Spark Blue™ 515 is best suited for detecting antigens with medium to high expression levels. It is also stable towards exposure to heat or a variety of commonly used fixative solutions.

Dyes similar to Spark Blue 515 based on emission

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5-CFDA 5-FAM AccuClear Aldefluor Alexa Fluor 488 ATTO 465 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 493/503 BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellTrace CFSE CellTracker Violet CF440 CF488A CF500 Chromeo 488 CoraLite Plus 488 CPN510 Green Cy2 CytoTrace Green CMFDA DiO DY-380XL DyLight 488 eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS510 FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) Live/Dead Fix Lime Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 Mag-Fura-2 MaxLight 490 mAzamiGreen MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 Nuclear Yellow NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PKH67 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Seta-385-NHS Spectrum Green STAR 440L STAR 440SXP StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green TFAX 488 SE TFAX 488 TFP T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Vio Bright 515 VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen CF498 Helix NP Green NucSpot 500/515 BDY FL Staurosporine Aluora 488 Mpro Tracer BactoSpore 485/500 Membrane Stain CytoLiner 495/510 mFluor Violet 510 BDY Lenalidomide Lysosome Probe Green CF505 RayBright Blue 488 Tricyclic cytosine tC Endoplasmic Reticulum Probe Green CoraLite 488 ABflo 500 ActinBrite 488/505 ABflo 488 AbBy Fluor 488 cFluor B515