YO-PRO-1
YO-PRO-1 (Ex-Max 491 nm/Em-Max 509 nm)
YO-PRO-1 is a fluorogenic, intercalating nucleic acid stain that is normally cell-impermeant to healthy cells but can be semi-permeant to cells undergoing apotosis due to the effects of a neutralization of the plasma membrane potential. It has an excitation peak at 491 nm and an emission peak at 509 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 491 nm
- Emission maximum
- 509 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
YO-PRO-1 has an excitation maximum at 491 nm and an emission maximum at 509 nm, a Stokes shift of 18 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is YO-PRO-1?
- YO-PRO-1 is a fluorogenic, intercalating nucleic acid stain that is normally cell-impermeant to healthy cells but can be semi-permeant to cells undergoing apotosis due to the effects of a neutralization of the plasma membrane potential. It has an excitation peak at 491 nm and an emission peak at 509 nm.
- What laser excites YO-PRO-1?
- YO-PRO-1 is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
- What filter should I use to detect YO-PRO-1?
- A 515/30 filter captures the peak of the YO-PRO-1 emission near 509 nm with minimal spillover.