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V500
V500 (Ex-Max 415 nm/Em-Max 500 nm)
V500 (Horizon V500) from BD Biosciences has an excitation peak at 415 nm and an emission peak at 500 nm. It is spectrally similar to AmCyan.
Excitation & emission spectra
Key facts
- Excitation maximum
- 415 nm
- Emission maximum
- 500 nm
- Optimal laser line
- 405
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 28500
- Commercial antibodies
- 69 from 1 supplier
V500 has an excitation maximum at 415 nm and an emission maximum at 500 nm, a Stokes shift of 85 nm. Its extinction coefficient is 28500 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is V500?
- V500 (Horizon V500) from BD Biosciences has an excitation peak at 415 nm and an emission peak at 500 nm. It is spectrally similar to AmCyan.
- What laser excites V500?
- V500 is optimally excited by the 405 laser line. Its excitation maximum is 415 nm.
- What filter should I use to detect V500?
- A 525/50 filter captures the peak of the V500 emission near 500 nm with minimal spillover.
- How bright is V500?
- V500 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to V500?
- FluoroFinder tracks 69 commercially available V500 conjugates, searchable by target, clone, host and application.