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Cy2

Cy2 (Ex-Max 492 nm/Em-Max 508 nm)

Cy2 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 492 nm and an emission peak at 508 nm. Spectrally its similar to fluorescein, Alexa Fluor™ 488 and CF®488A.

Excitation & emission spectra

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Key facts

Excitation maximum
492 nm
Emission maximum
508 nm
Optimal laser line
488
Optimal filter
515/30
Relative brightness
1 of 5
Molecular weight
665
Commercial conjugates
1 from 6 suppliers

Cy2 has an excitation maximum at 492 nm and an emission maximum at 508 nm, a Stokes shift of 16 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is Cy2?
Cy2 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 492 nm and an emission peak at 508 nm. Spectrally its similar to fluorescein, Alexa Fluor™ 488 and CF®488A.
What laser excites Cy2?
Cy2 is optimally excited by the 488 laser line. Its excitation maximum is 492 nm.
What filter should I use to detect Cy2?
A 515/30 filter captures the peak of the Cy2 emission near 508 nm with minimal spillover.
How bright is Cy2?
Cy2 has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to Cy2?
FluoroFinder tracks 1 commercially available Cy2 conjugates, searchable by target, clone, host and application.