Loading the Dye Directory…

7C

7C (Ex-Max 423 nm/Em-Max 499 nm)

7C from Novus/ Bio-Techne is a fluorophore with an excitation peak at 423 nm and an emission peak at 499 nm. It is an alternative to V500 or AmCyan in Flow Cytometry.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
423 nm
Emission maximum
499 nm
Optimal laser line
405
Optimal filter
515/30
Relative brightness
1 of 5
Commercial antibodies
2 from 1 supplier

7C has an excitation maximum at 423 nm and an emission maximum at 499 nm, a Stokes shift of 76 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is 7C?
7C from Novus/ Bio-Techne is a fluorophore with an excitation peak at 423 nm and an emission peak at 499 nm. It is an alternative to V500 or AmCyan in Flow Cytometry.
What laser excites 7C?
7C is optimally excited by the 405 laser line. Its excitation maximum is 423 nm.
What filter should I use to detect 7C?
A 515/30 filter captures the peak of the 7C emission near 499 nm with minimal spillover.
How bright is 7C?
7C has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to 7C?
FluoroFinder tracks 2 commercially available 7C conjugates, searchable by target, clone, host and application.