TRITC
TRITC (Ex-Max 557 nm/Em-Max 576 nm)
TRITC (Tetramethylrhodamine isothiocyanate) is a historical fluorophore from the rhodamine family that has an excitation peak at 557 nm and an emission peak at 576. Commonly used in fluorescence microscopy, brighter alternatives with better stability characteristics are Alexa Fluor™ 546, Alexa Fluor™ 568, SETA-555, Janelia Fluor® 549, iFluor® 555, eFluor™ 570, DyLight™ 550, CF®540, CF®550R and CF®555.
Excitation & emission spectra
Key facts
- Excitation maximum
- 557 nm
- Emission maximum
- 576 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Commercial antibodies
- 575 from 21 suppliers
TRITC has an excitation maximum at 557 nm and an emission maximum at 576 nm, a Stokes shift of 19 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is TRITC?
- TRITC (Tetramethylrhodamine isothiocyanate) is a historical fluorophore from the rhodamine family that has an excitation peak at 557 nm and an emission peak at 576. Commonly used in fluorescence microscopy, brighter alternatives with better stability characteristics are Alexa Fluor™ 546, Alexa Fluor™ 568, SETA-555, Janelia Fluor® 549, iFluor® 555, eFluor™ 570, DyLight™ 550, CF®540, CF®550R and CF®555.
- What laser excites TRITC?
- TRITC is optimally excited by the 532 laser line. Its excitation maximum is 557 nm.
- What filter should I use to detect TRITC?
- A 585/42 filter captures the peak of the TRITC emission near 576 nm with minimal spillover.
- How bright is TRITC?
- TRITC has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to TRITC?
- FluoroFinder tracks 575 commercially available TRITC conjugates, searchable by target, clone, host and application.