Seta-555-NHS
Seta-555-NHS (Ex-Max 555 nm/Em-Max 570 nm)
Seta-555 from SETA BioMedicals is a fluorophore with an excitation peak at 555 nm and an emission peak at 570 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 555 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 4 of 5
- Extinction coefficient
- 155000
- Quantum yield
- 0.07
Seta-555-NHS has an excitation maximum at 555 nm and an emission maximum at 570 nm, a Stokes shift of 15 nm. Its extinction coefficient is 155000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Seta-555-NHS?
- Seta-555 from SETA BioMedicals is a fluorophore with an excitation peak at 555 nm and an emission peak at 570 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
- What laser excites Seta-555-NHS?
- Seta-555-NHS is optimally excited by the 532 laser line. Its excitation maximum is 555 nm.
- What filter should I use to detect Seta-555-NHS?
- A 585/42 filter captures the peak of the Seta-555-NHS emission near 570 nm with minimal spillover.
- How bright is Seta-555-NHS?
- Seta-555-NHS has a relative brightness of 4 out of 5 (quantum yield 0.07).