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DyLight 549

DyLight™ 549 (Ex-Max 562 nm/Em-Max 576 nm)

DyLight™ 549 has been discontinued by the supplier and replaced by DyLight™ 550.

Excitation & emission spectra

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Key facts

Excitation maximum
562 nm
Emission maximum
576 nm
Optimal laser line
561
Optimal filter
585/42
Relative brightness
2 of 5
Commercial conjugates
4 from 6 suppliers

DyLight 549 has an excitation maximum at 562 nm and an emission maximum at 576 nm, a Stokes shift of 14 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What is DyLight 549?
DyLight™ 549 has been discontinued by the supplier and replaced by DyLight™ 550.
What laser excites DyLight 549?
DyLight 549 is optimally excited by the 561 laser line. Its excitation maximum is 562 nm.
What filter should I use to detect DyLight 549?
A 585/42 filter captures the peak of the DyLight 549 emission near 576 nm with minimal spillover.
How bright is DyLight 549?
DyLight 549 has a relative brightness of 2 out of 5.
How many antibodies are available conjugated to DyLight 549?
FluoroFinder tracks 4 commercially available DyLight 549 conjugates, searchable by target, clone, host and application.