CellBrite Fix 555
CellBrite Fix 555 (Ex-Max 542 nm/Em-Max 570 nm)
Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.
Excitation & emission spectra
Key facts
- Excitation maximum
- 542 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 1 supplier
CellBrite Fix 555 has an excitation maximum at 542 nm and an emission maximum at 570 nm, a Stokes shift of 28 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CellBrite Fix 555?
- Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.
- What laser excites CellBrite Fix 555?
- CellBrite Fix 555 is optimally excited by the 532 laser line. Its excitation maximum is 542 nm.
- What filter should I use to detect CellBrite Fix 555?
- A 585/42 filter captures the peak of the CellBrite Fix 555 emission near 570 nm with minimal spillover.
- How many antibodies are available conjugated to CellBrite Fix 555?
- FluoroFinder tracks 2 commercially available CellBrite Fix 555 conjugates, searchable by target, clone, host and application.