Dyes similar to CellBrite Fix 555 based on emission5-TAMRA 6-TAMRA Alexa Fluor 546 ATTO 542 ATTO 550 BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X BPE Brilliant Violet 570 BUV563 Calcein Red AM Calcein Red Calcium Orange CellBrite Orange CellBrite Steady 550 CellMask Orange Plasma Membrane Stain CellROX Orange CellTracker Orange CF535ST CF540 CF550R CF555 Chromeo 546 CMA-Chromomycin A3 CoraLite 555 Cy3 Cyanine 555 CytoTrace Red DiI DY-395XL DyLight 549 DyLight 550 Dyomics 547 eFluor 565NC eFluor 570 FVS570 FVS575V HiLyte 555 iFluor 555 Janelia Fluor 549 LSSmOrange LysoView 550 MaxLight 550 MemBrite Fix 543/560 MITO-ID Green MITO-ID MP (high potential) MitoLite Orange FX570 MitoTracker Orange mKusabira-Orange2 NeuroVue Orange Nile Red NL 557 NovaFluor Yellow 570 NucView 530 OC515 OFP Oyster 550 PE PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PKH26 POPO-3 PromoFluor 555P Pyronin Y Qdot 565 ReadiLink 555-570 Rhod-2 (Ca2+ bound) Rhod-5N (Ca2+ bound) Rhodamine B Rhodamine Seta-400-NHS Seta-555-NHS Spark Blue 574 Spark YG 570 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE SureLight RPE Synapto Green Sytox Orange TFAX 546 SE TMRE TRITC ViaKrome 561 VivaFix 547/573 VRDye 549 Zombie Yellow
About CellBrite Fix 555
Biotium’s CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium’s MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.