RFP
RFP (Ex-Max 558 nm/Em-Max 583 nm)
RFP has an excitation maximum at 558 nm and an emission maximum at 583 nm, a Stokes shift of 25 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Excitation & emission spectra
Key facts
- Excitation maximum
- 558 nm
- Emission maximum
- 583 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Extinction coefficient
- 100000
- Quantum yield
- 0.48
- Molecular weight
- 25900
RFP has an excitation maximum at 558 nm and an emission maximum at 583 nm, a Stokes shift of 25 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What laser excites RFP?
- RFP is optimally excited by the 561 laser line. Its excitation maximum is 558 nm.
- What filter should I use to detect RFP?
- A 585/42 filter captures the peak of the RFP emission near 583 nm with minimal spillover.