Seta-400-NHS
Seta-400-NHS (Ex-Max 399 nm/Em-Max 576 nm)
Seta-400 from SETA BioMedicals is a fluorophore with an excitation peak at 399 nm and an emission peak at 576 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 399 nm
- Emission maximum
- 576 nm
- Optimal laser line
- 405
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 24000
- Quantum yield
- 0.34
Seta-400-NHS has an excitation maximum at 399 nm and an emission maximum at 576 nm, a Stokes shift of 177 nm. Its extinction coefficient is 24000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Seta-400-NHS?
- Seta-400 from SETA BioMedicals is a fluorophore with an excitation peak at 399 nm and an emission peak at 576 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
- What laser excites Seta-400-NHS?
- Seta-400-NHS is optimally excited by the 405 laser line. Its excitation maximum is 399 nm.
- What filter should I use to detect Seta-400-NHS?
- A 585/42 filter captures the peak of the Seta-400-NHS emission near 576 nm with minimal spillover.
- How bright is Seta-400-NHS?
- Seta-400-NHS has a relative brightness of 2 out of 5 (quantum yield 0.34).