Live-or-Dye 568/583
Live-or-Dye™ 568/583 (Ex-Max 562 nm/Em-Max 584 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 568/583 has an excitation peak at 568 nm and an emission peak at 583 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 562 nm
- Emission maximum
- 584 nm
- Optimal laser line
- 488-561
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 568/583 has an excitation maximum at 562 nm and an emission maximum at 584 nm, a Stokes shift of 22 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is Live-or-Dye 568/583?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 568/583 has an excitation peak at 568 nm and an emission peak at 583 nm.
- What laser excites Live-or-Dye 568/583?
- Live-or-Dye 568/583 is optimally excited by the 488-561 laser line. Its excitation maximum is 562 nm.
- What filter should I use to detect Live-or-Dye 568/583?
- A 585/42 filter captures the peak of the Live-or-Dye 568/583 emission near 584 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 568/583?
- FluoroFinder tracks 2 commercially available Live-or-Dye 568/583 conjugates, searchable by target, clone, host and application.