532

Laser Line

586/14

Common Filter

554

Excitation Max

568

Emission Max

33,829

Antibodies

13

Conjugation Kits

1

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About Cy3


Cyanine 3 (Cy®3) is an orange-emitting fluorophore that can be excited by the 532 nm green laser and captured with a 586/14 nm bandpass filter. Cy3 has an excitation peak at 554 nm and an emission peak at 568 nm, and is spectrally similar to TRITC, Alexa Fluor 555, and DyLight 549. Cy3 is bright and can be a used for a variety of applications. Cy3 is most commonly used to label nucleic acids and is used for fluorescent microscopy. Cyanine dyes are known to be both photostable and resistant to harsh dehydration and embedding conditions that come with mounting tissue sections. While Cy3 can be used for flow cytometry, it is uncommon for flow cytometers to be equipped with 532 lasers. Additionally, Cy3 comes in a wide variety of modified formats that allow for easy conjugations. The Cyanine dye family are registered trademarks of GE Healthcare.


Popular Cy3 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
MilliporeSigma
AB5320C3
ICC,IHC NG2
Rat,Mouse,Human,Monkey
Rabbit
IgG
Polyclonal
100 μl
$389
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Biorbyt
orb114820-100ug
PROX1
Human,Mouse,Rat
Rabbit
IgG
100 μg
$451
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LifeSpan
LS-C704124-100
WB IL31RA
Human
Rabbit
IgG
100 μl
View
Bioss
bs-5575R-Cy3
IHC-P AMPK alpha (pS173)
Human,Mouse,Rat
Rabbit
IgG
100 μl
$481
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Bioss
bs-0647R-Cy3
FC,IHC-P CD4
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
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Bioss
bs-1009R-Cy3
IHC-P CD181
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
View
Bioss
bs-10529R-Cy3
IHC-P AOC3
Human,Mouse,Rat
Rabbit
IgG
100 μl
$457
View
Bioss
bs-21805R-Cy3
IF,IHC-P,IHC-Fr,ICC Clathrin Heavy Chain
Human,Mouse
Rabbit
IgG
100 μl
$485
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