tdTomato
tdTomato (Ex-Max 554 nm/Em-Max 581 nm)
tdTomato is a tandem dimer fluorescent protein derived from the coral anemones species Discosoma sp. It has an excitation peak at 554 nm and an emission peak at 581 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 554 nm
- Emission maximum
- 581 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Extinction coefficient
- 138000
- Quantum yield
- 0.69
- Molecular weight
- 54200
tdTomato has an excitation maximum at 554 nm and an emission maximum at 581 nm, a Stokes shift of 27 nm. Its extinction coefficient is 138000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is tdTomato?
- tdTomato is a tandem dimer fluorescent protein derived from the coral anemones species Discosoma sp. It has an excitation peak at 554 nm and an emission peak at 581 nm.
- What laser excites tdTomato?
- tdTomato is optimally excited by the 561 laser line. Its excitation maximum is 554 nm.
- What filter should I use to detect tdTomato?
- A 585/42 filter captures the peak of the tdTomato emission near 581 nm with minimal spillover.