532

Laser Line

575/36

Common Filter

556

Excitation Max

573

Emission Max

169

Antibodies

1

Conjugation Kits

1

Services


Spectra Viewer

About Alexa Fluor 546


Alexa Fluor® 546 (AF546, Alexa 546) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF532 has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor™ 555 (ATT Bioquest). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF532 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 546 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Novus Biologicals
DDXCH04A546-100
IF,FC,ICS, FC IgG4
Human
Human
IgG4
0.1 mg
$279
View
Novus Biologicals
DDX0300A546-100
ICC,IF CD123
Canine,Human
Mouse
IgG1
107D2.08
0.1 mg
$499
View
Novus Biologicals
DDX0700A546-100
ICC,IF CD127
Canine,Human
Mouse
IgG1
R34-34
0.1 mg
$499
View
Novus Biologicals
DDX0230A546-100
FC,ICC, IF PILR-alpha
Human
Rat
IgG2a
36H2
0.1 mg
$499
View
Thermo Fisher Scientific
A11035
IF,FC IgG
Rabbit
Goat
IgG
Polyclonal
500 μl
$199
View
Thermo Fisher Scientific
A20183
Labeling Alexa Fluor 546
N/A
N/A
N/A
5 x 100 μg
$473
View

DDX0700A546-100-0.05mg
ICC,IF CD127
Human,Canine
Mouse
IgG1
R34-34
50 μg
$299
View

DDX1434P-100AF546-0.05mg
FC,ICC, IF Integrin alpha 4 beta 7
Human
Mouse
IgG1
111D9.03
50 μg
$299
View
 Search Antibodies