CF540
CF®540 (Ex-Max 544 nm/Em-Max 570 nm)
CF®540 from Biotium is a fluorophore with an excitation peak at 544 nm and an emission peak at 570 nm. It can be useful in both fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 544 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
CF540 has an excitation maximum at 544 nm and an emission maximum at 570 nm, a Stokes shift of 26 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CF540?
- CF®540 from Biotium is a fluorophore with an excitation peak at 544 nm and an emission peak at 570 nm. It can be useful in both fluorescence microscopy and flow cytometry.
- What laser excites CF540?
- CF540 is optimally excited by the 532 laser line. Its excitation maximum is 544 nm.
- What filter should I use to detect CF540?
- A 585/42 filter captures the peak of the CF540 emission near 570 nm with minimal spillover.
- How bright is CF540?
- CF540 has a relative brightness of 2 out of 5.