10-Acetyl-37-dihydroxyphenoxazin
10-Acetyl-37-dihydroxyphenoxazin (Ex-Max 571 nm/Em-Max 584 nm)
10-Acetyl-37-dihydroxyphenoxazin (Amplex Red) is a fluorogenic substrate that is stoichiometrically converted in the presense horseradish peroxidase (HRP) and H2O2. This reagent is commonly used in Microplate Assay or to detect an HRP cojugated antibody.
Excitation & emission spectra
Key facts
- Excitation maximum
- 571 nm
- Emission maximum
- 584 nm
- Optimal laser line
- 561
- Optimal filter
- 586/14
- Extinction coefficient
- 54000
- Molecular weight
- 257
10-Acetyl-37-dihydroxyphenoxazin has an excitation maximum at 571 nm and an emission maximum at 584 nm, a Stokes shift of 13 nm. Its extinction coefficient is 54000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is 10-Acetyl-37-dihydroxyphenoxazin?
- 10-Acetyl-37-dihydroxyphenoxazin (Amplex Red) is a fluorogenic substrate that is stoichiometrically converted in the presense horseradish peroxidase (HRP) and H2O2. This reagent is commonly used in Microplate Assay or to detect an HRP cojugated antibody.
- What laser excites 10-Acetyl-37-dihydroxyphenoxazin?
- 10-Acetyl-37-dihydroxyphenoxazin is optimally excited by the 561 laser line. Its excitation maximum is 571 nm.
- What filter should I use to detect 10-Acetyl-37-dihydroxyphenoxazin?
- A 586/14 filter captures the peak of the 10-Acetyl-37-dihydroxyphenoxazin emission near 584 nm with minimal spillover.