DiI
DiI (Ex-Max 549 nm/Em-Max 567 nm)
DiL is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 549 nm and an emission peak at 567 nm
Excitation & emission spectra
Key facts
- Excitation maximum
- 549 nm
- Emission maximum
- 567 nm
- Optimal laser line
- 532
- Optimal filter
- 575/36
- Molecular weight
- 933.8793
DiI has an excitation maximum at 549 nm and an emission maximum at 567 nm, a Stokes shift of 18 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is DiI?
- DiL is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 549 nm and an emission peak at 567 nm
- What laser excites DiI?
- DiI is optimally excited by the 532 laser line. Its excitation maximum is 549 nm.
- What filter should I use to detect DiI?
- A 575/36 filter captures the peak of the DiI emission near 567 nm with minimal spillover.