OFP
OFP (Ex-Max 543 nm/Em-Max 567 nm)
OFP is an tetrameric orange fluorescent protein derived from the tube anemones, Ceriantharia. It has an excitation peak at 543 nm and an emission peak at 567 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 543 nm
- Emission maximum
- 567 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
OFP has an excitation maximum at 543 nm and an emission maximum at 567 nm, a Stokes shift of 24 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is OFP?
- OFP is an tetrameric orange fluorescent protein derived from the tube anemones, Ceriantharia. It has an excitation peak at 543 nm and an emission peak at 567 nm.
- What laser excites OFP?
- OFP is optimally excited by the 532 laser line. Its excitation maximum is 543 nm.
- What filter should I use to detect OFP?
- A 585/42 filter captures the peak of the OFP emission near 567 nm with minimal spillover.