LysoView 550
LysoView™ 550 (Ex-Max 542 nm/Em-Max 567 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 550 has an excitation peak at 542 nm and an emission peak at 567 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 542 nm
- Emission maximum
- 567 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 1 supplier
LysoView 550 has an excitation maximum at 542 nm and an emission maximum at 567 nm, a Stokes shift of 25 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is LysoView 550?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 550 has an excitation peak at 542 nm and an emission peak at 567 nm.
- What laser excites LysoView 550?
- LysoView 550 is optimally excited by the 532 laser line. Its excitation maximum is 542 nm.
- What filter should I use to detect LysoView 550?
- A 585/42 filter captures the peak of the LysoView 550 emission near 567 nm with minimal spillover.
- How many antibodies are available conjugated to LysoView 550?
- FluoroFinder tracks 2 commercially available LysoView 550 conjugates, searchable by target, clone, host and application.