Amplex Red
Amplex Red (Ex-Max 569 nm/Em-Max 581 nm)
10-Acetyl-37-dihydroxyphenoxazin (Amplex Red) is a fluorogenic substrate that is stoichiometrically converted in the presense horseradish peroxidase (HRP) and H2O2. This reagent is commonly used in Microplate Assay or to detect an HRP cojugated antibody in western blot. It has an excitation peak at 569 nm and an emission peak at 581 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 569 nm
- Emission maximum
- 581 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
Amplex Red has an excitation maximum at 569 nm and an emission maximum at 581 nm, a Stokes shift of 12 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Amplex Red?
- 10-Acetyl-37-dihydroxyphenoxazin (Amplex Red) is a fluorogenic substrate that is stoichiometrically converted in the presense horseradish peroxidase (HRP) and H2O2. This reagent is commonly used in Microplate Assay or to detect an HRP cojugated antibody in western blot. It has an excitation peak at 569 nm and an emission peak at 581 nm.
- What laser excites Amplex Red?
- Amplex Red is optimally excited by the 561 laser line. Its excitation maximum is 569 nm.
- What filter should I use to detect Amplex Red?
- A 585/42 filter captures the peak of the Amplex Red emission near 581 nm with minimal spillover.