About Amplex Red

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10-Acetyl-37-dihydroxyphenoxazin (Amplex Red) is a fluorogenic substrate that is stoichiometrically converted in the presense horseradish peroxidase (HRP) and H2O2. This reagent is commonly used in Microplate Assay or to detect an HRP cojugated antibody in western blot. It has an excitation peak at 569 nm and an emission peak at 581 nm.

Dyes similar to Amplex Red based on emission

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10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA alamarBlue Alexa Fluor 546 Alizarin Red ATTO 550 Calcein Red-Orange AM CellBrite Steady 550 CellTrace Yellow CF550R CF568 CF568ST CMA-Chromomycin A3 CytoTrace Red DsRed2 dsRed DsRed Express dTomato DY-510XL DyLight 549 DyLight 550 Dyomics 547 eFluor 585NC ExoBrite 560/585 EV Membrane Stain FVS570 Kaede (red) LipidSpot 488 Live-or-Dye 568/583 MagDot 575 MaxLight 550 MemBrite Fix 568/580 MITO-ID MP (high potential) MitoTracker Orange mScarlet mTangerine MultiDot 575 NeuroVue Red NL 557 NovaFluor Blue 585 NovaFluor Yellow 590 PE PhenoVue 512 Nucleic Acid Stain PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain pHrodo Red Qdot 585 Resazurin Resorufin RFP Rhod-2 (Ca2+ bound) Rhod-5N (Ca2+ bound) Rhodamine RealYellow 586 Seta-400-NHS SNARF 1 pH6.0 Spark YG 581 StarBright Blue 520 SureLight BPE SureLight RPE TagRFP tdTomato TMRE TRITC VivaFix 547/573 VRDye 549