dTomato
dTomato (Ex-Max 554 nm/Em-Max 581 nm)
dTomato is a dimeric fluorescent protein first isolated from sea anemone of the Discosoma genus with an excitation max at 554 nm and an emission max at 581 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 554 nm
- Emission maximum
- 581 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Extinction coefficient
- 69000
- Quantum yield
- 0.69
- Molecular weight
- 27000
dTomato has an excitation maximum at 554 nm and an emission maximum at 581 nm, a Stokes shift of 27 nm. Its extinction coefficient is 69000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is dTomato?
- dTomato is a dimeric fluorescent protein first isolated from sea anemone of the Discosoma genus with an excitation max at 554 nm and an emission max at 581 nm.
- What laser excites dTomato?
- dTomato is optimally excited by the 561 laser line. Its excitation maximum is 554 nm.
- What filter should I use to detect dTomato?
- A 585/42 filter captures the peak of the dTomato emission near 581 nm with minimal spillover.