Qdot 585
Qdot 585 (Ex-Max 300 nm/Em-Max 585 nm)
Qdot® 585 from Thermo Fisher Scientific is an inorganic nanocrystal with a surface functionalized to allow antibodies and biomolecules to be cross-linked. It is most efficiently excited between 320-405 nm but is capable of excitation across every laser less than its emission peak. It is significantly brighter and more photostable than traditional organic fluorophores, making it still useful for fluorescence microscopy, however its size and solubility issues make it a challenge to use in flow cytometry. In spectral flow cytometry it may still prove useful due to its very unique spectral characteristics
Excitation & emission spectra
Key facts
- Excitation maximum
- 355 nm
- Emission maximum
- 585 nm
- Optimal laser line
- 355, 405
- Optimal filter
- 585/42
- Relative brightness
- 3 of 5
- Commercial conjugates
- 2 from 1 supplier
Qdot 585 has an excitation maximum at 355 nm and an emission maximum at 585 nm, a Stokes shift of 230 nm. It is optimally excited by the 355, 405 laser line.
Frequently asked questions
- What is Qdot 585?
- Qdot® 585 from Thermo Fisher Scientific is an inorganic nanocrystal with a surface functionalized to allow antibodies and biomolecules to be cross-linked. It is most efficiently excited between 320-405 nm but is capable of excitation across every laser less than its emission peak. It is significantly brighter and more photostable than traditional organic fluorophores, making it still useful for fluorescence microscopy, however its size and solubility issues make it a challenge to use in flow cytometry. In spectral flow cytometry it may still prove useful due to its very unique spectral characteristics
- What laser excites Qdot 585?
- Qdot 585 is optimally excited by the 355, 405 laser line. Its excitation maximum is 355 nm.
- What filter should I use to detect Qdot 585?
- A 585/42 filter captures the peak of the Qdot 585 emission near 585 nm with minimal spillover.
- How bright is Qdot 585?
- Qdot 585 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to Qdot 585?
- FluoroFinder tracks 2 commercially available Qdot 585 conjugates, searchable by target, clone, host and application.