pHrodo Red
pHrodo Red (Ex-Max 560 nm/Em-Max 585 nm)
pHrodo Red from Thermo Fisher Scientific is a fluorogenic, cell-permeant pH indicator that is non-fluorescent at a neutral pH and increases in intensity with an increase in acidity involved in endocytosis and phagocytosis. PHrodo is often used to label proteins or biomolecules to track this process. PHrodo Red has an excitation peak at 560 nm and an emission peak at 585 nm, making it useful in both flow cytometry and fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 560 nm
- Emission maximum
- 585 nm
- Optimal laser line
- 488-561
- Optimal filter
- 585/40
- Commercial antibodies
- 1 from 1 supplier
pHrodo Red has an excitation maximum at 560 nm and an emission maximum at 585 nm, a Stokes shift of 25 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is pHrodo Red?
- pHrodo Red from Thermo Fisher Scientific is a fluorogenic, cell-permeant pH indicator that is non-fluorescent at a neutral pH and increases in intensity with an increase in acidity involved in endocytosis and phagocytosis. PHrodo is often used to label proteins or biomolecules to track this process. PHrodo Red has an excitation peak at 560 nm and an emission peak at 585 nm, making it useful in both flow cytometry and fluorescence microscopy.
- What laser excites pHrodo Red?
- pHrodo Red is optimally excited by the 488-561 laser line. Its excitation maximum is 560 nm.
- What filter should I use to detect pHrodo Red?
- A 585/40 filter captures the peak of the pHrodo Red emission near 585 nm with minimal spillover.
- How many antibodies are available conjugated to pHrodo Red?
- FluoroFinder tracks 1 commercially available pHrodo Red conjugates, searchable by target, clone, host and application.