RayBright PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 576 nm
- Optimal laser line
- 488, 532, 561
- Relative brightness
- 5 of 5
- Commercial antibodies
- 212 from 1 supplier
RayBright PE has an excitation maximum at 565 nm and an emission maximum at 576 nm, a Stokes shift of 11 nm. It is optimally excited by the 488, 532, 561 laser line.
Frequently asked questions
- What is RayBright PE?
- Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
- What laser excites RayBright PE?
- RayBright PE is optimally excited by the 488, 532, 561 laser line. Its excitation maximum is 565 nm.
- How bright is RayBright PE?
- RayBright PE has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RayBright PE?
- FluoroFinder tracks 212 commercially available RayBright PE conjugates, searchable by target, clone, host and application.