SNARF 1 pH6.0
SNARF 1 pH6.0 (Ex-Max 547 nm/Em-Max 584 nm)
SNARF-1, AM is a cell-permeant pH probe with a pKa of 7.5 after hydrolysis of the acetoxymethyl ester of the probe upon entering live cells. SNARF-1 is a ratiometric indicator sensitive to measuring pH changes between pH 7 and pH 8 and shifts from 580 nm under acidic pH to 640 nm under basic conditions. It also undergoes a shift in its excitation peak between pH6 to pH9 from ~532 nm at pH6 to ~561nm at pH9, but typically this probe is typically used in fluorescent imaging applications with a single excitation peak at 555nm to monitor the dual emission shift.
Excitation & emission spectra
Key facts
- Excitation maximum
- 547 nm
- Emission maximum
- 584 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
SNARF 1 pH6.0 has an excitation maximum at 547 nm and an emission maximum at 584 nm, a Stokes shift of 37 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is SNARF 1 pH6.0?
- SNARF-1, AM is a cell-permeant pH probe with a pKa of 7.5 after hydrolysis of the acetoxymethyl ester of the probe upon entering live cells. SNARF-1 is a ratiometric indicator sensitive to measuring pH changes between pH 7 and pH 8 and shifts from 580 nm under acidic pH to 640 nm under basic conditions. It also undergoes a shift in its excitation peak between pH6 to pH9 from ~532 nm at pH6 to ~561nm at pH9, but typically this probe is typically used in fluorescent imaging applications with a single excitation peak at 555nm to monitor the dual emission shift.
- What laser excites SNARF 1 pH6.0?
- SNARF 1 pH6.0 is optimally excited by the 532 laser line. Its excitation maximum is 547 nm.
- What filter should I use to detect SNARF 1 pH6.0?
- A 585/42 filter captures the peak of the SNARF 1 pH6.0 emission near 584 nm with minimal spillover.