CF568
CF®568 (Ex-Max 562 nm/Em-Max 583 nm)
CF®568 from Biotium is a fluorophore with an excitation peak at 562 nm and an emission peak at 583 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 568 and ATTO 565. However, in flow cytometry, a superior alternative is R-PE. In microscopy, CF®568 is validated in super-resolution imaging by STORM, STED, Structured Illumination (SIM) and TIRF.
Excitation & emission spectra
Key facts
- Excitation maximum
- 562 nm
- Emission maximum
- 583 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 100000
- Molecular weight
- 714
- Commercial antibodies
- 4.5k from 1 supplier
CF568 has an excitation maximum at 562 nm and an emission maximum at 583 nm, a Stokes shift of 21 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is CF568?
- CF®568 from Biotium is a fluorophore with an excitation peak at 562 nm and an emission peak at 583 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 568 and ATTO 565. However, in flow cytometry, a superior alternative is R-PE. In microscopy, CF®568 is validated in super-resolution imaging by STORM, STED, Structured Illumination (SIM) and TIRF.
- What laser excites CF568?
- CF568 is optimally excited by the 561 laser line. Its excitation maximum is 562 nm.
- What filter should I use to detect CF568?
- A 585/42 filter captures the peak of the CF568 emission near 583 nm with minimal spillover.
- How bright is CF568?
- CF568 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF568?
- FluoroFinder tracks 4492 commercially available CF568 conjugates, searchable by target, clone, host and application.