About MemBrite Fix 568/580

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Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.

Dyes similar to MemBrite Fix 568/580 based on emission

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10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA alamarBlue Alexa Fluor 555 Alizarin Red Amplex Red ATTO 550 ATTO 565 ATTO Rho3B Calcein Red-Orange AM CellBrite Steady 550 CellTrace Yellow CF550R CF568 CF568ST CF570 Cy3.5 CytoTrace Red DsRed2 dsRed DsRed Express dTomato DY-510XL DyLight 549 DyLight 550 eFluor 585NC EMA ExoBrite 560/585 EV Membrane Stain FVS570 GelRed Kaede (red) KikGR1 (red) LipidSpot 488 Live-or-Dye 568/583 Live-or-Dye NucFix Red MagDot 575 mApple MaxLight 550 MITO-ID MP (high potential) mScarlet mTangerine MultiDot 575 NeuroVue Red Nile Red NovaFluor Blue 585 NovaFluor Yellow 590 PE PhenoVue 512 Nucleic Acid Stain PhenoVue 577 Lysosomal Stain pHrodo Red PROTEOSTAT-Red Qdot 585 Resazurin Resorufin RFP Rhod-5N (Ca2+ bound) Rhodamine Rhodamine Red-X RY586 Seta-400-NHS SNARF 1 pH6.0 Spark YG 581 Spark YG 593 SureLight RPE Synapto Green TagRFP tdTomato TRITC VRDye 549