Dyes similar to MemBrite Fix 568/580 based on emission10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA alamarBlue Alexa Fluor 555 Alizarin Red Amplex Red ATTO 550 ATTO 565 ATTO Rho3B Calcein Red-Orange AM CellBrite Steady 550 CellTrace Yellow CF550R CF568 CF568ST CF570 CoraLite 568 Cy3.5 CytoTrace Red DsRed2 dsRed DsRed Express dTomato DY-510XL DyLight 549 DyLight 550 eFluor 585NC EMA ExoBrite 560/585 EV Membrane Stain FVS570 GelRed Kaede (red) KikGR1 (red) LipidSpot 488 Live-or-Dye 568/583 Live-or-Dye NucFix Red MagDot 575 mApple MaxLight 550 MITO-ID MP (high potential) mScarlet mTangerine MultiDot 575 NeuroVue Red Nile Red NovaFluor Blue 585 NovaFluor Yellow 590 PE PhenoVue 512 Nucleic Acid Stain PhenoVue 577 Lysosomal Stain pHrodo Red PROTEOSTAT-Red Qdot 585 Resazurin Resorufin RFP Rhod-5N (Ca2+ bound) Rhodamine Rhodamine Red-X RY586 Seta-400-NHS SNARF 1 pH6.0 Spark YG 581 Spark YG 593 SureLight RPE Synapto Green TagRFP tdTomato TRITC VRDye 549
About MemBrite Fix 568/580
Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.