SureLight BPE
SureLight B-PE (Ex-Max 545 nm/Em-Max 572 nm)
SureLight BPE from Columbia Biosciences is a fluorescent protein with an excitation peak at 545 nm and an emission peak at 572 nm. SureLight BPE is available in antibody conjugation kits for use in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 545 nm
- Emission maximum
- 572 nm
- Optimal laser line
- 488-561
- Optimal filter
- 576/36
- Relative brightness
- 5 of 5
- Extinction coefficient
- 2410000
- Molecular weight
- 240000
SureLight BPE has an excitation maximum at 545 nm and an emission maximum at 572 nm, a Stokes shift of 27 nm. Its extinction coefficient is 2410000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is SureLight BPE?
- SureLight BPE from Columbia Biosciences is a fluorescent protein with an excitation peak at 545 nm and an emission peak at 572 nm. SureLight BPE is available in antibody conjugation kits for use in flow cytometry.
- What laser excites SureLight BPE?
- SureLight BPE is optimally excited by the 488-561 laser line. Its excitation maximum is 545 nm.
- What filter should I use to detect SureLight BPE?
- A 576/36 filter captures the peak of the SureLight BPE emission near 572 nm with minimal spillover.
- How bright is SureLight BPE?
- SureLight BPE has a relative brightness of 5 out of 5.