TagRFP
TagRFP (Ex-Max 553 nm/Em-Max 581 nm)
TagRFP is a weakly dimeric fluorescent protein originally derived from the sea anemones, Entacmaea quadricolor. It has an excitation peak at 553 nm and an emission peak at 581 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 553 nm
- Emission maximum
- 581 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
TagRFP has an excitation maximum at 553 nm and an emission maximum at 581 nm, a Stokes shift of 28 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is TagRFP?
- TagRFP is a weakly dimeric fluorescent protein originally derived from the sea anemones, Entacmaea quadricolor. It has an excitation peak at 553 nm and an emission peak at 581 nm.
- What laser excites TagRFP?
- TagRFP is optimally excited by the 561 laser line. Its excitation maximum is 553 nm.
- What filter should I use to detect TagRFP?
- A 585/42 filter captures the peak of the TagRFP emission near 581 nm with minimal spillover.