561

Laser Line

585/42

Common Filter

561

Excitation Max

571

Emission Max


Spectra Viewer

About Calcein Red AM


Calcein Red is a red fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Red AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Red AM is hydrolyzed by esterase into the fluorescent Calcein Red which can then be measured via fluorescent microscopy, flow cytometer, or fluorescent microplate reader.