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Cyanine 3 SE

Cyanine 3 SE (Ex-Max 550 nm/Em-Max 560 nm)

Cyanine 3 has an excitation peak at 554 nm and an emission peak at 568 nm. Spectrally its similar to Alexa Fluor™ 555.

Excitation & emission spectra

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Key facts

Excitation maximum
550 nm
Emission maximum
560 nm
Optimal laser line
532
Optimal filter
575/36
Relative brightness
3 of 5
Extinction coefficient
150000
Quantum yield
0.31
Molecular weight
727.84

Cyanine 3 SE has an excitation maximum at 550 nm and an emission maximum at 560 nm, a Stokes shift of 10 nm. Its extinction coefficient is 150000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.

Frequently asked questions

What is Cyanine 3 SE?
Cyanine 3 has an excitation peak at 554 nm and an emission peak at 568 nm. Spectrally its similar to Alexa Fluor™ 555.
What laser excites Cyanine 3 SE?
Cyanine 3 SE is optimally excited by the 532 laser line. Its excitation maximum is 550 nm.
What filter should I use to detect Cyanine 3 SE?
A 575/36 filter captures the peak of the Cyanine 3 SE emission near 560 nm with minimal spillover.
How bright is Cyanine 3 SE?
Cyanine 3 SE has a relative brightness of 3 out of 5 (quantum yield 0.31).