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CellTracker Orange

CellTracker Orange (Ex-Max 541 nm/Em-Max 565 nm)

CellTracker® Orange CMRA is a fluorescent dye used for monitoring cell movement or location, allowing for multigenerational tracking of cellular movements. The blue excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
541 nm
Emission maximum
565 nm
Optimal laser line
532
Optimal filter
575/36

CellTracker Orange has an excitation maximum at 541 nm and an emission maximum at 565 nm, a Stokes shift of 24 nm. It is optimally excited by the 532 laser line.

Frequently asked questions

What is CellTracker Orange?
CellTracker® Orange CMRA is a fluorescent dye used for monitoring cell movement or location, allowing for multigenerational tracking of cellular movements. The blue excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.
What laser excites CellTracker Orange?
CellTracker Orange is optimally excited by the 532 laser line. Its excitation maximum is 541 nm.
What filter should I use to detect CellTracker Orange?
A 575/36 filter captures the peak of the CellTracker Orange emission near 565 nm with minimal spillover.