AcidiFluor ORANGE
AcidiFluor™ ORANGE is a fluorescence imaging probe which enhances fluorescence dramatically in acidic environments. This probe can stain acidic organelles such as lysosomes, late endosomes and granules selectively. It’s excellent selectivity enables detection of acidic environments. At pH 5.0, identical to the environment of acidic organelles, the fluorescence intensity is 50-fold or more compared to the intensity at pH 7.4.
Excitation & emission spectra
Full excitation and emission spectra are available in the interactive viewer.
Key facts
- Excitation maximum
- 535 nm
- Emission maximum
- 560 nm
- Optimal laser line
- 532
- Quantum yield
- .73
- Commercial antibodies
- 6 from 1 supplier
AcidiFluor ORANGE has an excitation maximum at 535 nm and an emission maximum at 560 nm, a Stokes shift of 25 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is AcidiFluor ORANGE?
- AcidiFluor™ ORANGE is a fluorescence imaging probe which enhances fluorescence dramatically in acidic environments. This probe can stain acidic organelles such as lysosomes, late endosomes and granules selectively. It’s excellent selectivity enables detection of acidic environments. At pH 5.0, identical to the environment of acidic organelles, the fluorescence intensity is 50-fold or more compared to the intensity at pH 7.4.
- What laser excites AcidiFluor ORANGE?
- AcidiFluor ORANGE is optimally excited by the 532 laser line. Its excitation maximum is 535 nm.
- How many antibodies are available conjugated to AcidiFluor ORANGE?
- FluoroFinder tracks 6 commercially available AcidiFluor ORANGE conjugates, searchable by target, clone, host and application.