CytoTrack Yellow
CytoTrack Yellow (Ex-Max 538 nm/Em-Max 559 nm)
CytoTrack Blue Yellow from Bio-Rad passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm. CytoTrack Yellow has an excitation peak at 538 nm and an emission peak at 559 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 538 nm
- Emission maximum
- 559 nm
- Optimal laser line
- 532
- Optimal filter
- 575/36
CytoTrack Yellow has an excitation maximum at 538 nm and an emission maximum at 559 nm, a Stokes shift of 21 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CytoTrack Yellow?
- CytoTrack Blue Yellow from Bio-Rad passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm. CytoTrack Yellow has an excitation peak at 538 nm and an emission peak at 559 nm.
- What laser excites CytoTrack Yellow?
- CytoTrack Yellow is optimally excited by the 532 laser line. Its excitation maximum is 538 nm.
- What filter should I use to detect CytoTrack Yellow?
- A 575/36 filter captures the peak of the CytoTrack Yellow emission near 559 nm with minimal spillover.