ATTO 542
ATTO 542 (Ex-Max 542 nm/Em-Max 562 nm)
ATTO 542 from ATTO-TEC has an excitation peak at 542 nm and an emission peak at 652 nm. ATTO 542 is a new fluorescent label related to ATTO 532. The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. After coupling to a substrate the dye carries a net electrical charge of - 3.
Excitation & emission spectra
Key facts
- Excitation maximum
- 542 nm
- Emission maximum
- 562 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 1 of 5
- Extinction coefficient
- 120000
- Quantum yield
- 0.93
- Fluorescence lifetime
- 3.7 ns
ATTO 542 has an excitation maximum at 542 nm and an emission maximum at 562 nm, a Stokes shift of 20 nm. Its extinction coefficient is 120000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is ATTO 542?
- ATTO 542 from ATTO-TEC has an excitation peak at 542 nm and an emission peak at 652 nm. ATTO 542 is a new fluorescent label related to ATTO 532. The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. After coupling to a substrate the dye carries a net electrical charge of - 3.
- What laser excites ATTO 542?
- ATTO 542 is optimally excited by the 532 laser line. Its excitation maximum is 542 nm.
- What filter should I use to detect ATTO 542?
- A 585/42 filter captures the peak of the ATTO 542 emission near 562 nm with minimal spillover.
- How bright is ATTO 542?
- ATTO 542 has a relative brightness of 1 out of 5 (quantum yield 0.93).